microarrays verigene  (BioFire Defense)

Journal: Annual review of analytical chemistry (Palo Alto, Calif.)

Article Title: Emerging Analytical Techniques for Rapid Pathogen Identification and Susceptibility Testing

doi: 10.1146/annurev-anchem-061318-115529

Figure Lengend Snippet: Summary of commercially available rapid identification/antibiotic susceptibility testing technologies for bloodstream infections. Adapted from Edmiston et al. ( 130 )

Article Snippet: 2.5 h Verigene Gram-Negative Blood Culture Luminex Blood culture Microarray CTX-M, IMI, VIM, KPC, NDM, OXA (Enterobacteriaceae) 2.5 h FilmArray Blood Culture identification BioFire Diagnostics Blood culture Nested PCR mecA ( Staphylococcus aureus ) vanA, vanB ( Enterococcus spp.)

Techniques: Multiplex Assay

Journal: Annual review of analytical chemistry (Palo Alto, Calif.)

Article Title: Emerging Analytical Techniques for Rapid Pathogen Identification and Susceptibility Testing

doi: 10.1146/annurev-anchem-061318-115529

Figure Lengend Snippet: List of NAAT platforms with genotypic resistance detection that are approved by the US Food and Drug Administration (FDA). Adapted from Bard & Lee ( 53 ) with permission from Elsevier

Article Snippet: 2.5 h Verigene Gram-Negative Blood Culture Luminex Blood culture Microarray CTX-M, IMI, VIM, KPC, NDM, OXA (Enterobacteriaceae) 2.5 h FilmArray Blood Culture identification BioFire Diagnostics Blood culture Nested PCR mecA ( Staphylococcus aureus ) vanA, vanB ( Enterococcus spp.)

Techniques: Luminex, Microarray, Nested PCR

Journal: Annual review of analytical chemistry (Palo Alto, Calif.)

Article Title: Emerging Analytical Techniques for Rapid Pathogen Identification and Susceptibility Testing

doi: 10.1146/annurev-anchem-061318-115529

Figure Lengend Snippet: Summary of commercially available rapid identification/antibiotic susceptibility testing technologies for bloodstream infections. Adapted from Edmiston et al. ( 130 )

Article Snippet: Due to these factors, phenotypic assays continue to be utilized as the gold standard for AST despite a lengthy assay time. table ft1 table-wrap mode="anchored" t5 caption a7 Assay Company Specimen type Method Resistance gene Run time Xpert MRSA/SA Gen 3 Cepheid Blood culture RT-PCR Methicillin ( mecA, mecC ) ~1 h Xpert MTB/RIF Cepheid Sputum RT-PCR rpoB ( Mycobacterium tuberculosis ) < 2 h Xpert MRSA/SA SSTI Cepheid Swab RT-PCR mecA ( Staphylococcus aureus ) ~1 h BD Max StaphSR Becton Dickinson Blood culture RT-PCR mecA ( Staphylococcus aureus ) ~1.5 h Verigene Gram-Positive Blood Culture Luminex Blood culture Microarray mecA ( Staphylococcus aureus ) vanA, vanB ( Enterococcus spp.)

Techniques: Multiplex Assay

Journal: Annual review of analytical chemistry (Palo Alto, Calif.)

Article Title: Emerging Analytical Techniques for Rapid Pathogen Identification and Susceptibility Testing

doi: 10.1146/annurev-anchem-061318-115529

Figure Lengend Snippet: List of NAAT platforms with genotypic resistance detection that are approved by the US Food and Drug Administration (FDA). Adapted from Bard & Lee ( 53 ) with permission from Elsevier

Article Snippet: Due to these factors, phenotypic assays continue to be utilized as the gold standard for AST despite a lengthy assay time. table ft1 table-wrap mode="anchored" t5 caption a7 Assay Company Specimen type Method Resistance gene Run time Xpert MRSA/SA Gen 3 Cepheid Blood culture RT-PCR Methicillin ( mecA, mecC ) ~1 h Xpert MTB/RIF Cepheid Sputum RT-PCR rpoB ( Mycobacterium tuberculosis ) < 2 h Xpert MRSA/SA SSTI Cepheid Swab RT-PCR mecA ( Staphylococcus aureus ) ~1 h BD Max StaphSR Becton Dickinson Blood culture RT-PCR mecA ( Staphylococcus aureus ) ~1.5 h Verigene Gram-Positive Blood Culture Luminex Blood culture Microarray mecA ( Staphylococcus aureus ) vanA, vanB ( Enterococcus spp.)

Techniques: Luminex, Microarray, Nested PCR